Oxygen sensing by T cells facilitates the establishment of cancer metastasis in the lung

Jan 26, 2019 10:01:56 AM


Dr. Nicholas Restifo’s group at the National Cancer Institute's Center for Cancer Research have discovered that oxygen-sensing proteins, called prolyl hydroxylase domain (PHD) proteins, act within T cells to prevent overly strong immune responses to harmless particles that frequently enter the lung. These PHD proteins promote the development of regulatory T cells and limit the development of inflammatory T cells.

The authors hypothesized that this protective mechanism may also allow circulating cancer cells to get a foothold in the lung. To test this, the researchers used PHD-knock-out mice. These PHD-knock-out mice, as well as normal mice, were injected with melanoma cells. Strikingly, whereas normal mice showed large amounts of cancerous melanoma cells in the lungs, the mice whose T cells lacked PHD proteins showed almost no evidence of melanoma in the lungs.

To accomplish this research, the authors used Bio X Cell’s anti-mouse IFNγ (clone XMG1.2) antibody to neutralize IFNγ-mediated anti-tumor immunity and confirm that PHD proteins limit IFNγ-mediated tumor clearance in the lung. Additionally, the authors used Bio X Cell’s anti-mouse IL-4 (clone 11B11) antibody to neutralize IL-4 and induce Th1 and Th17 cell differentiation in vitro.

See the article in Cell: http://www.cell.com/cell/abstract/S0092-8674(16)30982-5



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